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. 2022 Sep 19;16(12):2680–2690. doi: 10.1038/s41396-022-01322-8

Fig. 4. DAPG and pyoluteorin biosynthesis are affected by two independent signals from P. capeferrum F8.

Fig. 4

A Concentration of DAPG and pyoluteorin produced by P. protegens DTU9.1 cultured in KBmalt (control) or 50% v/v sterile-filtered supernatant from a 48 h culture of P. capeferrum F8. B Supernatant from a 48 h culture of P. capeferrum F8 was treated with proteinase K, boiled for 60 or 120 min at 100 °C or autoclaved for 15 min at 121 °C. P. protegens DTU9.1 harboring pSEVA237::PpltL-gfp was exposed to the treated supernatants, sterile water or fresh KBmalt media as control. Promoter activity was measured as the increase in relative fluorescence per h during stationary phase. C Concentration of DAPG and pyoluteorin produced by P. protegens DTU9.1 cultured in KBmalt supplemented with methanol as control or crude ethyl acetate extracted cell lysate of P. capeferrum F8. Significance levels were calculated with the Student’s t-test (*p < 0.05, **p < 0.005).