TABLE 3.
Specificity of CD8 T-cell clones A4, A10, and A11
| T-cell clone | Specific lysis in presence of indicated peptidea (%)
|
||||
|---|---|---|---|---|---|
| None | IYNVGQVSI | VYSLVFARL | CGPAVTTVGL | SYVPSAEQI | |
| None | 2 ± 2 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| A4 | 2 ± 2 | 80 ± 4 | 5 ± 4 | 9 ± 2 | 5 ± 4 |
| A10 | 13 ± 3 | 79 ± 6 | 20 ± 3 | 12 ± 5 | 12 ± 3 |
| A11 | 0 ± 0 | 82 ± 7 | 12 ± 5 | 14 ± 7 | 10 ± 3 |
a
A20J cells labeled with 51Cr were incubated with each peptide at a final concentration of 10−10 M. The assay was performed as described in Materials and Methods, using a T cell/target ratio of 5:1. Peptide IYNVGQVSI represents amino acids 359 to 367 encoded by TS gene 154 (44); VYSLVFARL and CGPAVTTVGL represent, respectively, amino acids 395 to 403 and 442 to 451 encoded by the same gene. The control peptide SYVPSAEQI is a CD8 epitope of the P. yoelii circumsporozoite protein that binds to H-2Kd (32). Results are expressed as the average of triplicate cultures ± SD.