Fig. 3. Burkholderia Lv-StB abundance throughout host development.

a Relative abundance of Burkholderia ASVs across different life stages based on Illumina sequencing of the 16 S rRNA gene V4 region compared to whole genome or MAG references of previously described strains. Each bar corresponds to a single individual, dissected organ of an adult female (“female gland”) or egg clutch. An asterisk (*) in the legend denotes pairwise identity above 98% but below 100%. b Lv-StB titers across host development in field and laboratory-raised individuals (Table S5) measured by qPCR with primers targeting the single-copy trans-AT PKS/NRPS lgaG gene involved in the biosynthesis of lagriamide by Lv-StB. Different letters indicate significant differences between life stages (Kruskal-Wallis χ2 = 97.712, df = 11, p value = 5.1 × 10⁻¹⁶, posthoc Dunn’s Test, α ≤ 0.05).