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. 2022 Nov 15;12:19584. doi: 10.1038/s41598-022-23855-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence for RPC transcription factors and cell cycle regulators in Müller glia during development. (A) Lhx2+ cells at P4 are mostly phospho-pRb (pRb) + RPC while those after P7 are pRb− Müller glia. (B) Pax6 immunoreactivity in Lhx2 + RPC and Müller glia (arrows). (C) Vsx2 immunoreactivity in Sox9 + RPC and Müller glia (arrows). (D) Nfia immunoreactivity in Lhx2 + RPC and Müller glia (arrows). (E) Cyclin D1 immunoreactivity in Lhx2 + RPC and Müller glia. (F) Cyclin D3 immunoreactivity in Lhx2 + RPC and Müller glia. NBL neuroblastic layer, GCL ganglion cell layer, ONL outer nuclear layer, INL inner nuclear layer. Scale bar = 20 µm.