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PNR-induced IL-8 gene expression is specific for the κB region of the IL-8 gene. PNR induces luciferase expression in A549 cells transfected with ×3 IL-8 κB site-luc. Relative luciferase activity was calculated from the luciferase activity of each reporter plasmid relative to the value of −50-luc, incubated with medium alone and assigned a value of 1. Mean fold increase relative to the untreated medium control was significantly higher in ×3 IL-8 κB site-luc than in −50-luc (P < 0.01).
