FIG. 5.

Induction of specific NF-κB complex formation by exposure of A549 cells to PNR. (A) Characterization of nuclear proteins bound to the NF-κB binding site of the IL-8 gene. Nuclear proteins were extracted from A549 cells cultured for 3 h without (lane 1) or with (lanes 2 to 5) PNR. EMSA was performed on nuclear extracts preincubated with no reagents (lanes 1 and 2) or the indicated competitors (lanes 3 to 5), and the NF-κB binding site of the IL-8 gene was used as a labeled probe. (B) Further characterization of nuclear proteins bound to the κB binding site of the IL-8 gene. Nuclear proteins were extracted from A549 cells that were cultured with PNR for 3 h. EMSA was performed on nuclear proteins preincubated with no reagents (lane 1) or the indicated antisera (lanes 2 to 5), and the κB binding site of the IL-8 gene was used as a labeled probe. Ab, antibody. Arrows indicate the specific complex.