Figure 1.
Time restricted feeding attenuates symptoms of autoinflammatory arthritis. (A) Schematic outline of the feeding regimens. (B) Short outline of the experimental setup and representative images of hind limbs of TRF and AL animals on the 6th day after arthritis induction. For further details, see Materials and Methods. Mice were injected with K/BxN arthritic serum i.p. on day 0. Arthritis development was followed by clinical scoring of the limbs (C) and ankle-thickness measurement (D). Data were normalized to values obtained on day 0 (mean ± SEM, n = 25 (AL) and n = 23 (TRF), repeated measures ANOVA, clinical score: group effect p = 0.053, group*day effect p = 0.0001, ankle-thickness: group effect p = 0.0056, group*day effect p = 0.0003, post-hoc Fisher test, * p < 0.05) (E) Neutrophil counts in the arthritic hind limbs of mice (mean + SEM, n = 7 (AL), n = 7 (TRF) mice, two-sample t-test, * p < 0.05).