TABLE 4.
Analysis of hemoprotein utilization by various Y. pestis hmu mutants
| Y. pestis strain | Relevant hmu characteristics
|
Hemoprotein utilization on PMHA-EDDA plates after 3 days at 37°Ca
|
||
|---|---|---|---|---|
| Chromosome | Plasmid | Growth | No growth | |
| KIM6+ | orfXY+ hmuP′RSTUV+ | None | He, Hb, Mb, Hb-Hp, He-alb, Hx | None |
| KIM6-2044.1+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | None | None | He, Hb, Mb, Hb-Hp, He-alb, Hx |
| KIM6-2044.1(pHMU4)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | orfY+ hmuP′RSTUV+ | He, Hb, Mb, Hb-Hp, He-alb, Hx | None |
| KIM6-2044.1(pHMU35)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | ′orfY hmuP′RSTUV+ | He, Hb | NT |
| KIM6-2044.1(pHMU37)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | orfY+ hmuP′RS+ hmuT′ | Hb, Hb-Hp, Hx | He, Mb, He-alb |
| KIM6-2044.1(pHMU30)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | hmuP′R+ | None | He, Hb, Mb, Hb-Hp, He-alb, Hx |
| KIM6-2044.1(pHMU51)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | hmuS+ hmuT′ | None | He, Hb, Mb, Hb-Hp, He-alb, Hx |
| KIM6-2044.1(pHMU30 + pHMU51)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | hmuP′R+ hmuS+ humT′ | Hb | He |
| KIM6-2044.1(pHMU66)+ | ΔorfXY ΔhmuP′RSTUV-2044.1 | orfY+ hmuP′R+ ΔhmuS hmuTUV+ | He, Hb, Mb, Hb-Hp, He-alb, Hx | None |
| KIM6-2061.1+ | In-frame ΔhmuR-2061.1 | None | None | He, Hb, Mb, Hb-Hp, He-alb, Hx |
| KIM6-2061.1(pHMU30)+ | In-frame ΔhmuR-2061.1 | hmuP′R+ | He, Hb, Mb, Hb-Hp, He-alb, Hx | None |
| KIM6-2062.1+ | In-frame ΔhmuS-2062.1 | None | He, Hb, Mb, Hb-Hp, He-alb, Hx(+/−) | None |
| KIM6-2063.1+ | hmuT::cat-2063.1 | None | Hb, Hx | He, Mb, Hb-Hp(?), He-alb |
Approximately 107 cells were overlaid onto deferrated PMH–100 μM EDDA agarose (PMHA-EDDA) plates. Twenty microliters of 500 μM hemin (He), 10 μM hemoglobin (Hb), 500 μM myoglobin (Mb), 100 μM hemoglobin-haptoglobin (Hb-Hp), 200 μM hemin-albumin (He-alb), or 100 μM heme-hemopexin (Hx) was added to 0.3-cm wells cut into the agarose, and growth around the wells was recorded after 3 days at 37°C. The concentrations of Hb-Hp (50% saturated), He-alb (50% saturated), and Hx (95% saturated) refer to the concentrations of the respective carrier proteins within the mixture. These results are based on at least three experiments. Hx(+/−), growth in two of three experiments; Hb-Hp(?), no growth in five of nine experiments; NT, no hemoproteins tested other than hemin and hemoglobin.