TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Host strain for cloning experiments | 58 |
| HB101 | Host strain essential for propagating plasmids carrying mutated H. ducreyi DNA inserts used in electroporation | 5, 58 |
| DH5α(pJL300) | Contains H. ducreyi 35000 cdtABC gene cluster; expresses H. ducreyi CDT activity | This study |
| DH5α(pJL303) | Contains H. ducreyi cdtABC gene cluster with mutated cdtC gene; expresses no CDT activity | This study |
| DH5α(pJL303)(pJL300-C) | DH5α(pJL303) also containing pJL300-C with the H. ducreyi cdtC gene; expresses CDT activity | This study |
| DH5α(pJL303)(pLS88) | DH5α(pJL303) also containing pLS88; expresses no CDT activity | This study |
| H. ducreyi | ||
| 35000 | Wild-type strain isolated in Winnipeg, Manitoba, Canada; produces CDT | 21 |
| 35000.303 | Isogenic cdtC mutant with a cat cartridge inserted into the cdtC gene; does not express CDT activity | This study |
| 35000.303(pJL300-C) | 35000.303 containing pJL300-C with the cdtC gene; expresses CDT activity | This study |
| 35000.303(pLS88) | 35000.303 containing pLS88; expresses no CDT activity | This study |
| Plasmids | ||
| pBR322 | Cloning vector; Ampr Tetr | 58 |
| pJL300 | pBR322 with a 3-kb H. ducreyi 35000 DNA insert containing the cdtABC gene cluster | This study |
| pJL303 | pJL300 with an cat cartridge inserted into the BseRI site within the cdtC gene | This study |
| pLS88 | Cloning vector capable of replication in H. ducreyi; Kanr Smr Sulr | 16 |
| pJL300-C | pLS88 with a 813-bp PCR-derived insert containing the H. ducreyi cdtC gene | This study |