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. 2022 Nov 2;13:977569. doi: 10.3389/fphys.2022.977569

FIGURE 3.

FIGURE 3

MDK 5220 in the KF decreased respiratory frequency, while ORX-B peptide affected both frequency and amplitude. (A) ORX-B (50 nl) was sufficient to decrease breathing frequency compared to pre-injection baseline, whereas MDK 5220 (100 nl) was needed to evoke a similar effect. Regardless of injection volume, vehicle injections did not change breathing frequency. (B) Only 100 nl ORX-B peptide injections lead to significant changes in amplitude. Each data point represents the normalized mean frequency (A) and amplitude (B) per injection site (mean of three consecutive 50 nl injections in the graphs on the left, and mean of two consecutive 100 nl injections in the graphs on the right). Bars and error bars are normalized group mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 with two-way ANOVA and Tukey’s post hoc tests.