Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Native-PAGE gel (5% stacking gel)
| Reagent | Final concentration | Amount |
|---|---|---|
| ddH2O | N/A | 1.35 mL |
| 30% acrylamide-bisacrylamide(29:1) | 5% | 335 μL |
| 1 M Tris-HCl (pH=6.8) | 125 mM | 250 μL |
| 10% APS | 0.1% | 20 μL |
| TEMED | 0.1% | 2 μL |
| Total | N/A | About 2 mL |
Mix well, pour into the rubber plate quickly and avoid bubbles. Insert the comb quickly. Store in fume hood for 30–60 min. Carry out the entire process in fume hood.
