Transfer buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-base | 26 mM | 3.13 g |
| Glycine | 200 mM | 14.4 g |
| Methanol | 20% | 200 mL |
| ddH2O | N/A | Add to 1 L |
| Total | N/A | 1 L |
Mix well, store at room temperature for at least half year.
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