REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CaIRF3 Rabbit mAb (1:5000)	Sun et al.6	N/A
Zebrafish LGP2 Rabbit mAb (1:5000)	Gong et al.1	N/A
β-Actin Rabbit mAb (1:5000)	Cell Signaling Technology	Cat# 8457; RRID: AB_10950489
HRP Goat Anti-Rabbit IgG (H+L) (secondary antibody) (1:5000)	ABclonal, China	Cat# AS014, RID: AB_2769854
Bacterial and virus strains
Spring viraemia of carp virus (SVCV)	Gong et al.1	N/A
Chemicals, peptides, and recombinant proteins
Protease inhibitor cocktail	Bimake	Cat# B14002
Phosphatase inhibitor cocktail	Bimake	Cat# B15001
poly(I:C)	SIGMA	Cat# P0913; CAS: 42424-50-0
RNAlater RNA stabilization reagent	QIAGEN	Cat# 76106
Methylene blue	Solarbio, China	Cat# M8030
MS-222	Alfa	Cat# 1809123
Crystal violet stain solution (1%)	Solarbio, China	Cat# G1062
Penicillin-streptomycin (100×), sterile	MedChemExpress	Cat# HY-K1006
RIPA lysis buffer (weak)	Beyotime, China	Cat# P0013D
Non-denaturing PAGE loading buffer (5×)	Beyotime, China	Cat# P0016
Sodium deoxycholate	Macklin, China	Cat# D6128; CAS: 302-95-4
Ammonium persulfate	Macklin, China	Cat# A801034; CAS: 7727-54-0
1 M Tris-HCl (pH=8.8)	Biosharp, China	Cat# BL515B
1 M Tris-HCl (pH=6.8)	Biosharp, China	Cat# BL514A
30% Acrylamide-bisacrylamide (29:1)	Biosharp, China	Cat# BL513B
N, N, N′, N′-Tetramethylethylenediamine (TEMED)	Beyotime, China	Cat# ST728
Glycin	Genview	Cat# FG149
Tris-base	Biofroxx	Cat# 1115KG001; CAS: 77-86-1
Polyethylenimine, linear (MW 25,000) (PEI)	Sigma-Aldrich	Cat# 23966-1
PBS	Monad	Cat# CR10601S
Tween-20	DING GUO, China	Cat# DH358-4
Clarity Western ECL substrate	BIO-RAD	Cat# 1705061
Methanol	SCR, China	Cat# 40064260; CAS: 67-56-1
Non-fat powdered milk	BBI, China	Cat# A600669
medium 199 (Gibco)	HyClone	Cat# SH30253.01
Fetal bovine serum (FBS)	WISENT	Cat# 086-150
Opti-mem	Invitrogen	Cat# 31985-070
Critical commercial assays
Endo-free Plasmid Mini Kit II	OMEGA	Cat# D6950-02
SV Total RNA Isolation	Promega	Cat# Z3100
TRIzol Plus RNA Purification kit	Invitrogen	Cat#12183555
GoScript™ Reverse Transcription System	Promega	Cat# A5001
Universal Blue qPCR SYBR Green Master Mix	YEASEN, China	Cat# 11201ES08
Dual-Luciferase® Reporter Assay System	Promega	Cat# P0913
Deposited data
Raw and analyzed data	This paper	Lead contact, Yibing Zhang (ybzhang@ihb.ac.cn)
Experimental models: Cell lines
Epithelioma papulosum cyprini cells (EPC)	ATCC (CRL-2872)	N/A
Ovary cells of grass carp (CO)	Kept in IHB, CAS	N/A
Crucian carp (C. auratus L.) blastulae embryonic cells (CAB)	Chen et al.7	N/A
Experimental models: Organisms/strains
Zebrafish (Danio rerio) strain AB: larvae (6 pdf) and adults (60 dpf, male: female =1:1)	China Zebrafish Resource Center	N/A
Recombinant DNA
pcDNA3.1(+)	Invitrogen	Cat# V79020
DrIFNφ1pro-luc (expression plasmid)	Sun et al.8	N/A
DrLGP2 (expression plasmid)	Gong et al.1	N/A
pRL-TK (expression plasmid)	Promega	Cat# E2241
Software and algorithms
GraphPad Prism 8.0	Dotmatics	https://www.graphpad.com/
Other
Mini-PROTEAN® tetra system	BIO-RAD	Cat# 1658004
Mini Trans-Blot® Electrophoretic Transfer Cell	BIO-RAD	Cat# 170-3930
Mini-PROTEAN® system casting stand	BIO-RAD	Cat# 1658050
LUMINESCENT IMAGE ANALYZER	LISTED	Model: ImageQuant LAS 4000 mini
24-well plate	LABSELECT, China	Cat# 11320
RNase-free microcentrifuge tubes	BBI, China	Cat# F611541-0001
Animal dissection forceps	Fine Science Tools	Cat# 11295-10
Animal anatomical scissors	Easybio, China	CAT#BE6621
Ceramic beads, 1.4 mm (325 g)	QIAGEN	CAT#13113-325