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. 2022 Oct 27;13:1034774. doi: 10.3389/fimmu.2022.1034774

Table 1.

Binding kinetics of interactions between soluble HER2 and immobilized HCT-mono-mIL12, as measured by bio-layer interferometry.

	Binding kinetic parameters
	k _on (M^-1s^-1, ×10⁵)	k _off (s^-1, ×10^-3)	K _D (nM)	R ^2(a)	k _on ratio	k _off ratio	K _D ratio
	k _on (M^-1s^-1, ×10⁵)	k _off (s^-1, ×10^-3)	K _D (nM)	R ^2(a)	(variant vs. HCT/0.5-mono-mIL12)^(b)
HCT	3.63 ± 0.10	0.13 ± 0.02	0.36 ± 0.06	0.99	–	–	–
HCT/0.5-mono-mIL12	3.27 ± 0.11	0.18 ± 0.02	0.54 ± 0.08	0.99	1	1	1
HCT/46-mono-mIL12	2.14 ± 0.06	9.85 ± 0.13	46.1 ± 1.46	0.99	0.65	55	85
HCT/130-mono-mIL12	1.45 ± 0.06	18.90 ± 0.54	130.0 ± 6.48	0.99	0.54	105	241
HCT/217-mono-mIL12	1.34 ± 0.08	29.20 ± 0.67	217.0 ± 13.90	0.99	0.46	162	402

^(a)Correlation coefficient value for the fitting.

^(b)The ratio was obtained versus the corresponding value of HCT/0.5-mono-mIL12.