REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-PSD95 (1:400 dilution)	Abcam	Catalog# AB12093
Mouse anti-AT8 (1:250 dilution)	Invitrogen	Catalog# MN1020
Mouse anti-CD43 (1:25 dilution)	Invitrogen	Catalog# 14-0439-82
Mouse anti-CD45 (1:40 dilution)	Invitrogen	Catalog# 14-9457-82
Mouse anti-CD68 (1:100 dilution)	Invitrogen	Catalog# MA5-13324
Rabbit anti-CD235 (1:100 dilution)	Invitrogen	Catalog# PA5-27154
Rabbit anti-Ferritin (1:250 dilution)	MilliporeSigma	Catalog# F6136
Rabbit anti-Iba1 (1:100 dilution)	Wako	Catalog# 019-19741
Mouse anti-Ki67(1:200 dilution)	Thermo Fisher Scientific	Catalog# RM-9106-S
Rabbit anti-TMEM119 (1:200 dilution)	Invitrogen	Catalog# PA5-62505
Donkey anti-goat 488 (1:500 dilution)	Invitrogen	Catalog# A11055
Donkey anti-goat 594 (1:500 dilution)	Invitrogen	Catalog# A11058
Donkey anti-goat 647 (1:500 dilution)	Invitrogen	Catalog# A21447
Goat anti-mouse 488 (1:500 dilution)	Invitrogen	Catalog# A11029
Goat anti-mouse 594 (1:500 dilution)	Invitrogen	Catalog# A11032
Goat anti-mouse 647 (1:500 dilution)	Invitrogen	Catalog# A21235
Goat anti-rabbit 488 (1:500 dilution)	Invitrogen	Catalog# A27034
Goat anti-rabbit 594 (1:500 dilution)	Invitrogen	Catalog# A11037
Goat anti-rabbit 647 (1:500 dilution)	Invitrogen	Catalog# A21245
Biological samples
Human brain tissue samples	UCI-ADRC	See Table 1 for detailed information
Chemicals, peptides, and recombinant proteins
2-Mercaptoethanol	Gibco	Catalog# 21985-023
Adult brain dissociation kit	Miltenyi Biotec	Catalog# 130-107-677
BMP4	Peprotech	Catalog# 120-05ET
Chroman 1	MedChem Express	Catalog# HY-15392
DPBS	Cytiva HyClone	Catalog# SH30028.02
DMEM/F12	Cytiva HyClone	Catalog# SH3002201
DNase I recombinant	Roche	Catalog# 4536282001
Emricasan	SelleckChem	Catalog# S7775
GlutaMAX	Gibco	Catalog# 35050-061
IL-3	Peprotech	Catalog# 200-03
Corning® Matrigel® hESC-Qualified Matrix	MilliporeSigma	Catalog# CLS354277
M-CSF	Peprotech	Catalog# 300-25
Mouse cell depletion cocktail	Miltenyi Biotec	Catalog# 130-104-694
mTeSR 1	Stemcell Technologies	Catalog# 85850
mTeSR plus	Stemcell Technologies	Catalog# 100-0276
Polyamine supplement (1000×)	MilliporeSigma	Catalog# P8483
ReLeSR	Stemcell Technologies	Catalog# 100-0484
SCF	Peprotech	Catalog# 300-07
Trans-ISRIB	R&D Systems	Catalog# 5284
VEGF	Peprotech	Catalog# 100-20
X-VIVOTM 15	Lonza	Catalog# 04-418Q
Y-27632	Tocris	Catalog# 1254
Ketamine	Covetrus	Catalog# 11695-0703-1
Xylazine	Covetrus	Catalog# 11695-4024-1
Bupivacaine	MP Biomedicals	Catalog# 154904
Penicillin-Streptomycin	Cytiva HyClone	Catalog# SV30010
Critical commercial assays
Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1	10x Genomics	Catalog# 1000128
Chromium Next GEM Chip G Single Cell Kit	10x Genomics	Catalog#1000127
Single Index Kit T Set A	10x Genomics	Catalog#1000213
Deposited data
scRNA-seq data		NCBI GEO: GSE189227
Experimental models: Cell lines
Cell lines	Coriell Institute for Medical Research	See Table 2 for detailed information
Experimental models: Organisms/strains
Mice: Rag2−/−hCSF1	The Jackson Laboratory	IMSR_JAX:017708 Genotype: C;129S4-Rag2tm1.1FlvCsf1tm1(CSF1)FlvIl2rgtm1.1Flv/J Age: P0 Sex: both
Software and algorithms
Adobe Illustrator	Adobe	https://www.adobe.com/products/illustrator.html
Adobe Photoshop	Adobe	https://www.adobe.com/products/photoshop.html
BioRender	BioRender.Inc	https://biorender.com/
Cell Ranger v6.1.1	10x Genomics	https://github.com/10XGenomics/cellranger
Cluster Profiler 4.0.5		https://guangchuangyu.github.io/software/clusterProfiler/
Clampfit 10.5	Molecular Devices	https://support.moleculardevices.com/s/
GraphPad Prism 9.2.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ	NIH	https://imagej.net/software/fiji/
Imaris 9.5.1	Bitplane	https://imaris.oxinst.com
Zen 2.3	Carl Zeiss	N/A
Other
Digital stereotaxic device	David KOPF	Model 940
Mouse and Neonates Adaptor	Stoeling	Catalog# 51625
Syringe 1701RN	Hamilton	Catalog# 7653-01
Small Hub RN Needle	Hamilton	Catalog# nc1767272
GentleMACS Octo Diss. with Heating Units	Miltenyi Biotec	Catalog# 130-096-427
QuadroMACS Separator	Miltenyi Biotec	Catalog# 130-091-051
Chromium X	10×Genomics	Catalog# PN-1000323
High Speed Rotary Micromotor Kit	Foredom	Catalog# K.1070
Micro Drill Bit, Uncoated	Kyocera	Catalog# 07289, #77 (Bit Size)
LS Columns	Miltenyi Biotec	Catalog# 130-042-401
MidiMACS Separator	Miltenyi Biotec	Catalog# 130-042-302
70 μm cell strainer	Bio TC	Catalog# C4070BTC