Table 1.

Thirty-five most functionally stable PAI-1 variants relative to WT

Mutation	Functional stability score	padj	Massively parallel t1/2, h
I91L	3.73	2.27 × 10−188	ND
L169S	3.67	6.25 × 10−29	ND
I91F	3.47	<10−294	7.7
F372V	3.39	<10−294	6.1
D222H	3.22	5.14 × 10−136	ND
I91M	3.20	5.51 × 10−165	ND
S331G	2.97	<10−294	6.8
D222Y	2.95	4.76 × 10−293	ND
Y241R	2.94	2.66 × 10−26	ND
M45K	2.82	<10−294	3.9
G332S	2.81	4.63 × 10−242	6.2
T333A	2.74	<10−294	7.8
Y221C	2.72	5.47 × 10−240	ND
F372I	2.72	<10−294	4.0
G332D	2.70	9.24 × 10−225	7.0
R271L	2.67	3.20 × 10−239	3.7
K154I	2.62	1.21 × 10−266	3.3
G332A	2.62	2.48 × 10−124	ND
F372C	2.57	8.19 × 10−123	ND
D222V	2.56	1.54 × 10−263	ND
E378D	2.45	7.76 × 10−236	4.9
G332R	2.44	1.60 × 10−55	ND
T323I	2.40	<10−294	ND
L272R	2.38	1.95 × 10−193	3.4
K176E	2.38	1.26 × 10−210	4.1
D222N	2.37	<10−294	2.0
F372L	2.34	<10−294	3.7
T333S	2.33	8.77 × 10−65	8.4
Y241H	2.31	<10−294	2.0
V334G	2.28	1.37 × 10−89	ND
S198C	2.26	4.44 × 10−118	ND
G332V	2.18	1.80 × 10−70	7.6
W86L	2.17	9.72 × 10−274	13.1
R271C	2.16	4.50 × 10−277	3.9
L169H	2.14	1.68 × 10−88	3.2

Abbreviation: ND, not determined because of insufficient data.

p_adj = adjusted p value as defined by Love et al. (61).