Table 1.
Neutralizing activities of plasma IgG and VRC01-like mAbs
| Mouse ID or mAb | Adjuvant | 426c.WT (293T) | 426c.WT (GnTI-) | 426c.TM (GnTI-) | 426c.TM.D279K (GnTI-) | 426c.SM (293T) | 426c.SM (GnTI-) |
|---|---|---|---|---|---|---|---|
| M4 | Poly(I:C) | >100 | >100 | 0.92 | >100 | NT | NT |
| M5 | >100 | >100 | 0.79 | 88.27 | NT | NT | |
| M6 | >100 | >100 | 0.84 | 88.09 | NT | NT | |
| M13 | GLA-LSQ | >100 | >100 | 3.53 | >100 | NT | NT |
| M14 | >100 | >100 | 1.83 | >100 | NT | NT | |
| M15 | >100 | >100 | 0.39 | >100 | NT | NT | |
| M16 | >100 | >100 | 0.14 | >100 | NT | NT | |
| M21 | Rehydragel | >98.5 | >98.5 | 0.84 | >98.5 | NT | NT |
| M22 | >84.5 | >84.5 | 0.48 | >84.5 | NT | NT | |
| M23 | >72 | >72 | 6.08 | >72 | NT | NT | |
| M24 | >81.9 | >81.9 | >81.9 | >81.9 | NT | NT | |
| mVRC01 | 2.47 | 0.18 | <0.005 | >10.00 | NT | NT | |
| MLK-002 | Poly(I:C) | >51.85 | >51.85 | <0.02 | NT | >17.33 | 1.724 |
| MLK-008 | GLA-LSQ | >50 | >50 | <0.02 | NT | 7.13 | 0.2 |
| MLK-009 | >50 | >50 | <0.02 | NT | NT | NT | |
| MLK-010 | >50 | >50 | <0.02 | NT | 2.748 | 0.13 | |
| MLK-014 | >50 | >50 | 0.05 | NT | >16.67 | 0.26 | |
| MLK-015 | >21 | >21 | <0.01 | NT | 2.89 | 0.06 | |
| MLK-016 | >50 | >50 | <0.02 | NT | 10.52 | 0.17 | |
| mVRC01 | 1.77 | 0.17 | <0.0023 | NT | 0.38 | 0.05 | |
| glVRC01 | >50 | >50 | 0.78 | NT | >33.33 | 0.81 |
See also Figures S2 and S3. IgG was isolated from plasma collected 2 weeks following the booster immunization with HxB2.WT.Core from the indicated mouse in the different adjuvant groups and was evaluated for the presence of neutralizing antibodies against the WT 426c virus and the 426c virus whose Env lacks three NLGS (N276, N460 and N463), TM, and against its variant with the D279K mutation that abrogates the neutralizing activity of VRC01 antibodies. Viruses were expressed in 293 GnTI- cells. The WT 426c virus was also expressed in 293T cells. Values represent IC50 neutralization values in μg/mL. Neutralization IC50 values of these same viruses with the mature VRC01 mAb are included. VRC01-like mAbs isolated after the boost immunization with HxB2.WT.Core from the Poly(I:C) and GLA-LSQ groups (see Figure 5 for binding information) were evaluated against the same viruses as the plasma samples as well as against the 426c variant whose Env only lacks the N276 NLGS (SM) expressed in 293T and 293 GnTI- cells. Values represent IC50 neutralization values in mg/mL. Neutralization IC50 values of these same viruses with the mature and germline VRC01 mAbs are included. Bold values indicate samples displaying neutralizing activity. NT indicates samples not tested.