REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC Rat Anti-Mouse IgG1 (clone A85-1)	BD Biosciences	Cat# 553443; RRID:AB_394862
FITC Rat Anti-Mouse IgG2b (clone R12-3)	BD Biosciences	Cat# 553395; RRID:AB_394833
Goat antiMouse IgG2c:FITC	Bio-Rad	Cat# STAR135F; RRID:AB_1102667
FITC Rat Anti-Mouse IgG3 (clone R40-82)	BD Biosciences	Cat# 553403; RRID:AB_394840
PerCP/Cy5.5 anti-mouse IgD Antibody	Biolegend	Cat# 405710; RRID:AB_1575113
eBioscience Fixable Viability Dye eFluor 506	ThermoFisher Scientific	65-0866-14
BV510 Hamster Anti-Mouse CD3e (clone 145-2c11)	BD Biosciences	Cat# 563024; RRID:AB_2737959
BV510 Rat Anti-Mouse CD4 (clone RM4-5)	BD Biosciences	Cat# 563106; RRID:AB_2687550
Brilliant Violent 510 anti-mouse F4/80 Antibody	Biolegend	Cat# 123135; RRID:AB_2562622
Brilliant Violet 605 anti-mouse IgM Antibody	Biolegend	Cat# 406523; RRID:AB_2563358
BV786 Rat Anti-Mouse CD45R/B220	BD Bioscience	Cat# 563894; RRID:AB_2738472
BV650 Rat Anti-Mouse CD19	BD Biosciences	Cat# 563235; RRID:AB_2738085
6x-His Tag Monoclonal Antibody (HIS.H8)	ThermoFisher Scientific	Cat# MA1-21315; RRID:AB_557403
HRP Goat anti-mouse IgG	Biolegend	Cat# 405306; RRID:AB_315009
Bacterial and virus strains
NEB® 5-alpha Competent E. coli (High Efficiency)	New England BioLabs	C2987H
HIV-1 Env-pseudotyped viruses	D. Montefiori and C. LaBranche, Duke University	N/A
Chemicals, peptides, and recombinant proteins
Poly (I:C)	InvivoGene	tlrl-pic-5
GLA-LSQ	Infectious Disease Reseach Institute (IDRI)	IDRI-LS130
Rehydragel	NIAID	N/A
Steptavidin-R-Phycoerythirin	Prozyme	PJRS25
Streptavidin-Allophycocyanin	Prozyme	PJ27S
RNaseOUT™ Recombinant Ribonuclease Inhibitor	ThermoFisher Scientific	10777019
HotStar Taq Plus	Qiagen	203607
Gel Red Nucleic Acid Stain	Biotium	41002
ExoSap-IT	Affymetrix	78201
SureBlue Reserve TMB Microwell Peroxidase Substrate	KPL	53-00-02
293-Free Transfection Reagent	Millipore Sigma	72181
5x In-Fusion enzyme	Takara Bio	1805251A
SuperScript IV RT	ThermoFisher Scientific	18090200
Critical commercial assays
EasySep Mouse B Cell Isolation Kit	Stemcell Technologies	19854
InFusion HD Cloning Kit	Takara Bio	639649
QIAprep Spin Miniprep Kit	Qiagen	27106
britelite plus Reporter Gene Assay System	PerkinElmer	6066766
Thrombin, Restriction Grade	EMD Millipore	69671-3
Deposited data
Antibody Sequence	This paper	Table S2
Experimental models: Organisms/strains
Mouse: glH-VRC01	Jardine et al., 2015	N/A
Oligonucleotides
Random Primers	ThermoFisher Scientific	48190011
GeneAmp dNTP Blend	ThermoFisher Scientific	N8080261
Primers for PCR	This paper; Jardine et al., 2015; Tiller et al., 2008	Table S1
Recombinant DNA
Ptt3 k vector	Snijder et al., 2018	N/A
PMN 4-341 vector	Mouquet et al., 2012	N/A
Software and algorithms
Geneious (Version 8.1.9)	Biomatters Ltd.	https://www.geneious.com
IMGT/V-QUEST	Brochet et al., 2008; Giudicelli et al., 2011	http://www.imgt.org/IMGT_vquest/input
Prism 7	GraphPad	https://www.graphpad.com
FlowJo_V10	BD	https://www.flowjo.com
Other
Anti-PE microbeads	Miltenyi Biotech	130-048-801
Anti-APC microbeads	Miltenyi Biotech	130-090-855
LS Columns	Miltenyi Biotech	130-042-401
Pierce Protein A agarose beads	ThermoFisher Scientific	18-5063
Anti-human IgG Fc Capture (AHC) Biosensors	ForteBio	18-5126
MyOne Tosylactivated Dynabeads	ThermoFisher Scientific	65501
His60 Ni-Superflow Resin	Takara Bio	636660
HiLoad 16/600 Superdex 200 pg (GE) column	GE Healthcare	28989335
Superose 6 10/300 GL	GE Healthcare	17-6172-01
Agarose Bound Galanthus Nivalis Lectin	Vector Labs	AL-1243-5
Step-Tactin SuperFlow Plus	Qiagen	30002
HisTrap FF Column	GE Healthcare	17525501