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| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-base | 50 mM | 6.06 g |
| NaCl | 500 mM | 29.25 g |
| DTT | 2 mM | 0.31 g |
| Glycerol | 5% | 50 mL |
Adjust the pH of the buffer to 7.5 at 25°C. Add Milli-Q water up to 1 L and filter the buffer using a 0.22 μm filter. The buffer can be stored at 4°C for 1 day.
