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. 2022 Nov 16;289(1987):20221642. doi: 10.1098/rspb.2022.1642

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© 2022 The Author(s)

Published by the Royal Society. All rights reserved.

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Figure 5. — Ageing-associated immune dysregulation and immunopathology. (a) Malpighian tubule (MT) activity (n = 12–20 females/infection treatment/age group), as a proxy for immunopathological damage, measured at 4 h after infection with 0.1 OD of P. rettgeri. Statistically significant difference between groups are indicated by asterisk (*). (b) Expression of positive (Relish, PGRP-LC) and negative (Caudal, Pirk) regulators of Imd pathway across sexes and age groups after P. rettgeri infection, relative to an internal control rp49 (n = total 15–21 flies homogenized in Trizol in a group of 3/Infection treatment/age group/sex-combination). n.s. = not significant. (Online version in colour.)