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. Author manuscript; available in PMC: 2022 Nov 16.
Published in final edited form as: Cell Rep. 2017 Dec 19;21(12):3548–3558. doi: 10.1016/j.celrep.2017.11.081

Figure 2. Expression, Activity, and Localization of mRNA-Encoded MUT.

Figure 2.

Human fibroblasts (1 × 106) isolated from a healthy subject (control) and 2 mut0 patients were transfected with 1 μg eGFP or hMUT mRNA for 24 hr.

(A) hMUT expression in transfected human fibroblasts was determined by western blotting. Expression levels were quantified and normalized to α-tubulin levels.

(B) MUT activity in transfected human fibroblasts.

(C) Transfected cells were incubated with MitoTracker Red for 30 min to mark mitochondria and then stained with an anti-MUT mouse monoclonal antibody (mAb) to examine cellular localization (scale bars, 10 μm).

Data are presented as mean ± SD. *p < 0.05 compared to corresponding control group of same treatment, #p < 0.05 compared to its own eGFP group. p values obtained from pairwise Tukey’s post hoc test after two-way ANOVA. n = 3 independent experiments.