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. Author manuscript; available in PMC: 2022 Nov 16.
Published in final edited form as: Cell Rep. 2017 Dec 19;21(12):3548–3558. doi: 10.1016/j.celrep.2017.11.081

Figure 3. Pharmacokinetics of LNP and hMUT mRNA and Protein.

Figure 3.

Wild-type mice (CD-1) were administered a single i.v. bolus of 0.5 mg/kg hMUT mRNA encapsulated in a biodegradable LNP and sacrificed at various time points (2, 6, 16 hr, 1, 2, 3, 5, 7 days; n = 3 mice/time point).

(A) Ionizable lipid in liver extracts was quantified by LC-MS/MS.

(B) Hepatic hMUT mRNA following hMUT administration was quantified using a branched-chain DNA (bDNA) assay.

(C) hMUT mRNA distribution was determined by in situ hybridization (ISH) using an hMUT mRNA-specific probe.

(D) Hepatic hMUT protein levels following hMUT mRNA administration. hMUT protein was quantified by LC-MS/MS.

****p < 0.0001, p values obtained from Tukey’s multiple-comparison test after one-way ANOVA.