. 2022 Oct 31;5(11):1142–1155. doi: 10.1021/acsptsci.2c00162

Table 1. Equilibrium Dissociation Constants (pK_d values) of the Fluorescent Y₁R Ligand 1 Obtained from BRET Saturation Binding Experiments.

compound	receptor (construct)	pK_d (BRET)^a	N
1	Nluc-Y₁R(Nterm)	n.a.	–
	Nluc-Y₁R(Δ1–31)	8.54 ± 0.05	3
	Nluc-Y₁R(Y192)	9.62 ± 0.05	4
	Nluc-Y₁R(Q291)	9.54 ± 0.07	4

Determined by BRET saturation binding experiments at intact HEK293T cells stably expressing the respective receptor construct. Data are shown as means ± SEM of N independent experiments performed in triplicate. n.a.: not applicable.

Table 1. Equilibrium Dissociation Constants (pKd values) of the Fluorescent Y1R Ligand 1 Obtained from BRET Saturation Binding Experiments.

Table 1. Equilibrium Dissociation Constants (pK_d values) of the Fluorescent Y₁R Ligand 1 Obtained from BRET Saturation Binding Experiments.