Table 2. Thermal Shift and ITC Data for Aminobenzothiazole Analogues 2 and 87–89 against p53-Y220C.

^aMeasured by DSF using 8 μM protein and 10× SYPRO Orange.

^bMeasured by ITC (reverse titrations) using 4–15 μM ligand and 100–147 μM protein. ΔT_m and K_d values were calculated as the average of at least three independent measurements.

^cConsistent with literature value ΔT_m = 2.0 °C ([L] = 250 μM).

^dLit.⁴²K_d = 4.1 μM.