Fig. 2.

ARPC1B promotes the self-renewal and IR resistance of GSCs in vitro. A Representative images and quantification of tumor sphere formation of GSCs transduced with two different sh-ARPC1B sequences. Scale bar, 100 μm. B Limiting dilution assays of MES GSCs expressing sh-control or sh-ARPC1B and PN GSCs expressing vector or ARPC1B. C Flow cytometric analysis showing the impact of ARPC1B knockdown on the apoptosis of GSCs treated with IR (6 Gy). The right panels show the quantification of the apoptosis rate. D Representative images and quantification of comet assays showing the effect of ARPC1B knockdown on DNA damage in GSCs treated with IR (6 Gy). Scale bar, 20 μm