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. 2022 Nov 16;41:324. doi: 10.1186/s13046-022-02514-y

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© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Comparison of aerotaxis versus intrinsic migration of breast cancer cells. 24 h trajectories of 50 representative cells per condition followed as XY plots (scales in µm) of the indicated cells either (A) plated at 5% confluency (intrinsic migration) or (B) subjected to the aerotactic assay (see also Figure S2 for T5, T6, T8 T10 and T16 cells). (C) Mean values of speed ± SD (in µm/min) of the indicated cells (10 A stands for MCF10A). (D) Mean relative distance of migration ± SD (in µm) of the indicated cells at 24 h. (E) Mean directionality ± SD (no unit) is given for aerotaxis (blue) and intrinsic (carmine) cell migration. (F) Graphical representation of the intrinsic speed of tumour cells as a function of the relative distance travelled in the aerotactic test. Pearson’s correlation coefficient is given. (All of the data displayed are representative of at least 3 independent experiments)