FIG. 4.

Functional comparison of isogenic CNA variants. Each of the isogenic cna clones was introduced into the cna-negative strain UAMS-128 (U128) and assayed by FACS (A) and quantitative collagen binding assay (B). Results of FACS are reported as mean fluorescence intensity (MFI); results of ¹²⁵I-collagen binding assays are reported as disintegrations per minute (dpm). Results shown represent the average of two experiments with each experiment including duplicate assays. Results are shown as mean ± standard error of the mean. (C) Cumulative results of all collagen binding assays (n = 30). (D) Results of shear force studies. Units on the x axis are per second. Results shown represent the average of two assays, each of which was repeated twice. Error bars indicate standard error of the mean. The asterisks in panels C and D indicate statistical significance (P < 0.05).