Fig. 4.

Gating scheme and cell populations for FACS selections. The sorted sample contained 5 × 10⁶ anti-cmyc antibody-labeled yeast cells, 1 × 10⁶ Violet Cell Trace^™ dye-labeled target-expressing mammalian cells, and 2 × 10⁶ unlabeled target-null mammalian cells. Six populations arose from this cell mixture: (1) unbound scFv-expressing yeast cells; (2) yeast-bound target-null cells; (3) yeast-bound target-expressing cells; (4) unbound non-expressing yeast cells; (5) unbound target-null cells; and (6) unbound target-expressing cells. The upper right quadrant contains the yeast-bound target-expressing cells, which represents the desired sort population