TABLE 1.

Plasmids used in this study

Plasmid	Description a	Source/reference
YEp352	Multi‐copy empty vector	19
pHsNHA2	H. sapiens NHA2 in YEp352	This work
pGRU1	Multi‐copy empty vector with GFP	B. Daignan‐Fornier, NCBI Acc. No. AJ249649
pHsNHA2‐GFP	H. sapiens NHA2 in pGRU1	This work
pGFP‐HsNHA2	N‐terminally GFP tagged HsNHA2 in YEp352	This work
pHsNHA2t	H. sapiens NHA2 with TPS1 terminator in YEp352	This work
pGFP‐HsNHA2t	N‐terminally GFP tagged HsNHA2 with TPS1 terminator in YEp352	This work
pNHA1‐985	S. cerevisiae NHA1‐985 in YEp352	20
pNHA1‐985GFP	S. cerevisiae NHA1‐985 in pGRU1	20
pTEF1‐HsNHA2‐HA	H. sapiens NHA2 in p426TEF	4
pRS316	Centromeric empty vector	21
pC‐HsNHA2t	H. sapiens NHA2 with TPS1 terminator in pRS316	This work
pC‐GFP‐HsNHA2t	N‐terminally GFP tagged HsNHA2 with TPS1 terminator in pRS316	This work
YEp352‐ZrSTL1‐TPS1t‐kanMX	Plasmid for the expression of Zygosaccharomyces rouxii STL1 gene downstream with the TPS1 terminator	22

^a ScNHA1 or HsNHA2 were expressed under the control of S. cerevisiae NHA1 promoter, except in pTEF1‐HsNHA2‐HA, in which TEF1 promoter was used.