TABLE 1.
C. parvum GSSs matched to known sequences from C. parvum and other organisms in public databasesa
| Function and clone name | Accession no. of closest hit | Description | Organism | P |
|---|---|---|---|---|
| Cell division | ||||
| CpGR24B | gb/U69154 | Prohibitin | Nicotiana tabacum | 2.80e-53 |
| Cell envelope | ||||
| CpGR102A | gb/Z22537 | Precursor of oocyst wall | Cryptosporidium parvum | 2.30e-14 |
| Cellular metabolism | ||||
| Biosynthesis of cofactors | ||||
| CpGR327A | sp/P22217 | Thioredoxin II (TR-II) | Saccharomyces cerevisiae | 6.30e-24 |
| Energy metabolism | ||||
| CpGR27A/B/69A/B/195 | gb/U89342 | Phosphoglucomutase | Zea mays | 3.80e-54 |
| A/B/336A | ||||
| CpGR160A | pir/S58236 | Pyruvate oxidoreductase | Entamoeba histolytica | 1.70e-31 |
| CpGR230A/B | gb/AB000703 | Phosphomannomutase | Schizosaccharomyces pombe | 5.20e-11 |
| CpGR245A | gb/D84307 | Phosphor-ethanol-amine cytidylyl-transferase | Homo sapiens | 2.4e-32 |
| Fatty acid and phospholipid metabolism | ||||
| CpGR306A | gb/U85829 | Enolase gene | Spongilla sp. | 1.10e-28 |
| CpGR496A | sp/P14685 | Probable diphenol oxidase A2 components | Mus musculus | 3.9e-22 |
| CpGR452A | gb/D82928 | Phosphatidylinositol synthase | Rattus norvegicus | 1.2e-06 |
| Purines or pyrimidines | ||||
| CpGR240A | pir/S69219 | Pseudouridine synthase 2 | Saccharomyces cerevisiae | 8.00e-23 |
| CpGR458A | gb/U15181 | Deoxyuridine 5′ triphosphate nucleotidohydrolase | Mycobacterium leprae | 2.1e-06 |
| CpGR437A | sp/P36590/ | Thymidylate kinase | Schizosaccharomyces pombe | 8.8e-27 |
| Cell signaling | ||||
| Ligand | ||||
| CpGR17B | pir/A55053 | Endothelial monocyte-activating protein | Caenorhabditis elegans | 7.60e-11 |
| Receptor and their associated proteins | ||||
| CpGR7B, CpGR385A | gb/U12596 | TNF type 1 receptor associated protein | Homo sapiens | 4.10e-48 |
| CpGR24A | gb/U28940 | B-cell receptor associated protein | Homo sapiens | 3.30e-14 |
| CpGR26A/B | sp/P48643, sp/P40413 | T-complex protein 1, Epsilon subunit | Saccharomyces cerevisiae | 2.30e-52 |
| CpGR159A | gb/S67127/S67127 | Endothelin ETA receptor | Homo sapiens | 8.30e-10 |
| Protein kinase and phosphatase | ||||
| CpGR21A | gb/D50927 | KIAA0137 gene product related to protein kinase | Rattus norvegicus | 9.5e-27 |
| CpGR192B | pir/S19027 | Protein kinase A catalytic chain | Aplysia californica | 2.50e-23 |
| CpGR312A | sp/P07312 | Casein kinase II, beta chain | Bos taurus | 2.10e-35 |
| CpGR425A | pir/S39559 | Mitogen-activated protein kinase | Nicotiana tabacum | 5.70e-27 |
| CpGR302A/B | pir/A55661 | Protein kinase ADK1 | Arabidopsis thaliana | 1.20e-85 |
| CpGR360A | gb/U78721 | Protein phosphatase 2C | Arabidopsis thaliana | 2.30e-12 |
| CpGR494A | pir/I38215 | Protein-serine/threonine kinase | Homo sapiens | 1.1e-24 |
| Other | ||||
| CpGR231B | gb/U59684 | Shk1 kinase-binding protein | Schizosaccharomyces pombe | 4.00e-10 |
| CpGR176A | sp/P35447 | F-spondin precursor | Xenopus laevis | 4.3e-06 |
| CpGR260A | sp/P35446 | F-spondin precursor | Rattus norvegicus | 5.1e-14 |
| CpGR455A | pir/I38176 | ragA | Homo sapiens | 1.6e-24 |
| CpGR352A | gb/U23449 | Diacylglycerol kinase | Caenorhabditis elegans | 1.80e-23 |
| CpGR44B | sp/P53742 | Possible GTP-binding protein | Saccharomyces cerevisiae | 1.60e-54 |
| Development | ||||
| CpGR263A | gb/D87957 | Protein involved in sexual development | Homo sapiens | 1.2e-61 |
| DNA replication and metabolism | ||||
| Degradation of DNA | ||||
| CpGR355A | sp/P12638 | Endonuclease IV | Escherichia coli | 1.90e-52 |
| DNA replication, restriction, modification, recombination, and repair | ||||
| CpGR98A | sp/P41004 | Chromosome segregation protein | Homo sapiens | 3.90e-14 |
| CpGR376A | sp/P49005 | DNA polymerase delta small subunit | Homo sapiens | 3.40e-10 |
| CpGR299A | sp/P49643 | DNA primase 58-kDa subunit | Homo sapiens | 5.5e-05 |
| CpGR453A | gb/Z99167 | Hypothetical helicase | Schizosaccharomyces pombe | 3.1e-10 |
| CpGR468A | sp/P32908 | Chromosome segregation protein | Saccharomyces cerevisiae | 5.3e-16 |
| CpGr465A | sp/O12749 | DNA repair protein RHC18 | Saccharomyces cerevisiae | 1.4e-11 |
| CpGR152A | emb/X81813 | Small subunit of DNA polymerase delta | Saccharomyces cerevisiae | 1.9e-08 |
| CpGR433A | pir/S67922 | Telomeric DNA binding protein 1 | Homo sapiens | 8.3e-10 |
| Intracellular trafficking | ||||
| CpGR157A | gb/Z68880 | Coat protein gamma-COP-bovine | Caenorhabditis elegans | 9.40e-15 |
| CpGR457A | sp/P11442 | Clathrin heavy chain | Rattus norvegicus | 3.0e-53 |
| CpGR454A | pir/S52426 | s-SNAP protein | Loligo pealei | 5.6e-15 |
| CpGR277A | sp/P35200 | Small chain of the clathrin-assembly proteins | Saccharomyces cerevisiae | 1.3e-12 |
| CpGR179B | gb/U81030 | Treacle | Mus musculus | 1.2e-09 |
| CpGR42B/310A/B/36A | pir/S51683 | Organelle heat shock protein 70 | Eimeria tenella | 1.30e-89 |
| Membrane transport | ||||
| CpGR211A | sp/P23787 | Transitional ER ATPase | Xenopus laevis | 2.10e-35 |
| CpGR236A/B | pir/S71261 | V-type proton-ATPase | Saccharomyces cerevisiae | 2.00e-36 |
| CpGR222A | sp/P38735 | Probable ATP-dependent permease | Saccharomyces cerevisiae | 1.50e-11 |
| CpGR216A | sp/p39109 | Metal resistance protein YCF1 | Saccharomyces cerevisiae | 6.8e-13 |
| Protein synthesis and degradation | ||||
| Ribosomal proteins | ||||
| CpGR61A | sp/P35687 | 40S ribosomal protein S21 | Oryza sativa | 4.10e-23 |
| CpGR67B | pir/S67197 | Ribosomal protein S10 | Saccharomyces cerevisiae | 3.30e-27 |
| CpGR62B | pir/B48470 | Ubiquitin-ribosomal protein fusion | Saccharomyces cerevisiae | 3.00e-78 |
| CpGR223B | gb/L16558 | Ribosomal protein L7 | Homo sapiens | 5.40e-48 |
| CpGR229B | sp/P12947 | 60S ribosomal protein L31 | Homo sapiens | 1.30e-34 |
| CpGR168B | sp/P17702 | 60S ribosomal protein L28 | Rattus norvegicus | 1.3e-06 |
| Aminoacyl-tRNA synthetase, tRNAs, and their modification | ||||
| CpGR17B | gb/U89436 | Tyrosyl-tRNA synthetase | Homo sapiens | 1.3e-14 |
| CpGR123A | gb/Z85984 | Histidyl tRNA synthetase | Homo sapiens | 4.20e-42 |
| Posttranslational modification | ||||
| CpGR290A | sp/P50579 | Methionine aminopeptidase 2 | Homo sapiens | 1.4e-06 |
| CpGR295A | sp/O63009 | Protein arginine N-methyltransferase | Rattus norvegicus | 1.6e-75 |
| Protein modification and translation factors | ||||
| CpGR70A/B | gb/U71180 | Elongation factor 1 alpha | Cryptosporidium parvum | 2.50e-131 |
| CpGR357A | gb/D21163 | Elongation factor 2 | Homo sapiens | 1.70e-27 |
| CpGR438A | gb/AB002753 | Elongation factor 1 alpha | Entamoeba histolytica | 2.4e-36 |
| CpGR183A/B | gb/U48261 | Protein disulfide isomerase | Cryptosporidium parvum | 2.90e-110 |
| Degradation of proteins | ||||
| CpGR147A/B | pir/S35971 | Aspartic proteinase | Eimeria acervulina | 3.60e-37 |
| CpGR7B | gb/D78151 | 26S proteasome subunit | Homo sapiens | 3.30e-33 |
| CpGR212A | sp/P12881 | Proteasome 29-kDa subunit | Drosophila melanogaster | 1.50e-61 |
| CpGR221A/B | gb/Y09505 | Proteasome delta subunit | Nicotiana tabacum | 6.80e-28 |
| CpGR165B/118B | sp/P52488 | Ubiquitin-activating enzyme E1 | Saccharomyces cerevisiae | 1.00e-17 |
| CpGR234B | gb/Z25704 | Ubiquitin-conjugating enzyme | Arabidopsis thaliana | 1.10e-17 |
| CpGR489A | sp/P50101 | Putative ubiquitin carboxyl-terminal hydrolase | Saccharomyces cerevisiae | 4.6e-57 |
| CpGR461A | sp/P45181 | Probable zinc protease PQQL | Haemophilus influenzae | 3.4e-07 |
| Transcription and mRNA regulation | ||||
| CpGR395A | sp/P28370 | Possible global transcription activator | Homo sapiens | 1.20e-35 |
| CpGR141A | pir/A54964 | Spliceosome-associated protein SA | Homo sapiens | 3.60e-29 |
| CpGR194B/198B | sp/P21675 | Transcription initiation factor I | Homo sapiens | 1.3e-07 |
| CpGR473A | gb/AC002332 | Putative pre-mRNA splicing factor | Arabidopsis thaliana | 2.8e-14 |
| CpGR235B | sp/Q08111 | Nitrogen regulation protein NIFR3 | Saccharomyces cerevisiae | 1.00e-19 |
| CpGR228A | gb/X95455 | Ring zinc finger protein | Gallus gallus | 2.8e-05 |
| CpGR461A | sp/P45181 | Probable zinc protein | Haemophilus influenzae | 3.4e-07 |
| Dead box proteins | ||||
| CpGR2A/249A/B | sp/P42305 | Dead box protein, RNA helicase | Bacillus subtilis | 4.70e-15 |
| CpGR10A/372A | sp/P53131 | Putative ATP-dependent RNA helicase | Saccharomyces cerevisiae | 1.60e-71 |
| CpGR10B | gb/U13644 | Pre-mRNA splicing factor RNA helicase, DEAD subfamily | Caenorhabditis elegans | 7.10e-25 |
| CpGR14A | sp/P25808 | ATP-dependent RNA helicase | Saccharomyces cerevisiae | 1.20e-18 |
| CpGR73B | gb/U80447 | Dead box protein | Caenorhabditis elegans | 5.10e-23 |
| CpGR6A | gb/X95906 | Cleavage and polyadenylation specificity factor protein | Bos taurus | 7.40e-35 |
| CpGR233A | pir/A56236 | Probable RNA helicase 1 | Homo sapiens | 6.60e-48 |
| Cytoskeleton | ||||
| CpGR427A | pir/A25342 | Tubulin beta chain | Cryptosporidium parvum | 4.60e-63 |
| CpGR33A/B | gb/D50929 | KiAA0139 gene product related to mouse controsomin B | Homo sapiens | 8.80e-26 |
| CpGR396A | sp/P10587 | Myosin heavy chain | Gallus gallus | 1.0e-08 |
| Hypothetical proteins | ||||
| CpGR44A | gb/L05425 | Autoantigen | Homo sapiens | 2.60e-59 |
| CpGR91A/92A | sp/P24212 | SBMA protein | Escherichia coli | 2.00e-18 |
| CpGR140A | gb/U50078 | p619 | Homo sapiens | 5.70e-10 |
| CpGR164A | gb/Z70757 | ZK287.5 | Caenorhabditis elegans | 3.10e-11 |
| CpGR177A/194A | gb/U80437 | C43E11.9 | Caenorhabditis elegans | 9.40e-46 |
| CpGR238B | gb/U41540 | Coded for by C. elegans | Caenorhabditis elegans | 3.70e-12 |
| CpGR383A | gb/M59420 | C. parvum DNA segment B | Cryptosporidium parvum | 4.10e-111 |
| CpGR394A/408A | gb/U18112 | C. parvum ORF2 gene | Cryptosporidium parvum | 4.70e-37 |
| CpGR404A | sp/P36148/ | Hypothetical 83.6-kDa protein in CCP1-SIS2 | Saccharomyces cerevisiae | 3.30e-11 |
| CpGR420A | gb/L29389 | Fun12p | Saccharomyces cerevisiae | 6.20e-43 |
| CpGR191A | gb/X96698 | D1075-like gene product | Homo sapiens | 6.0e-05 |
| CpGR289A | gb/X98253 | ZNF183 | Homo sapiens | 2.9e-14 |
| CpGR460A | pir/S51431 | Hypothetical protein YLR186w | Saccharomyces cerevisiae | 3.4e-06 |
| CpGR181A | pir/A57640 | Retinoblastoma protein-binding protein | Homo sapiens | 3.1e-06 |
| CpGR466A | pir/S68689 | Glucose regulated protein | Cricetulus griseus | 6.1e-07 |
| CpGR0493A | gb/AF017267 | Thrombospondin related | Cryptosporidium parvum | 4.2e-20 |
| rRNA and tRNA genes | ||||
| CpGR12A/B | gb/AF040725 | 5.8S/16S/18S rRNA gene | Cryptosporidium parvum | 0 |
| CpGR0309B | tRNAscan-SEb | Ile tRNA | Thiobacillus ferrooxidans | NAc |
a
List of GSSs sharing similarities (P ≤ 10−5) with previously reported sequences from GenBank (gb), SWISS-PROT (sp), and PIR (pir). The GSSs are sorted according to their functional categories based on the classification system developed by Riley (27). A complete and more detailed table is available (5, 35).
b
Identified by the program tRNAscan-SE.
c
NA, not applicable.