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. Author manuscript; available in PMC: 2022 Nov 16.
Published in final edited form as: Methods Mol Biol. 2022;2429:233–246. doi: 10.1007/978-1-0716-1979-7_15

Fig. 2.

Fig. 2

Representative immunofluorescence images showing phenotypic markers for specific cell types during embryonic origin-specific VSMC differentiation. (a–k) The time point labeled on each set of cell marker-positive fluorescence images is consistent with that shown on the bright-field image of the same cell type in Fig. 1. (l–o) Embryonic origin-specific VSMCs are positive for TAGLN and CNN1 after being cultured in the SMC growth medium for 14 days. Scale bars represent 100 μm