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Effect of PRPP on PyrR–BL2 RNA interaction. PyrR (1 nM) and BL2 RNA (50 pM) concentrations were held constant. The concentration of PRPP was increased from 0 to 500 µM in the presence of no cofactor (filled circles), 200 µM UMP (filled squares) or 200 µM UTP (filled triangles).
