Table 2.

Primers used in the RT-qPCR experiment.

Bacterial strain	Protein (gene)	Primers	References
Str. mitis	Biofilm-associated protein (bapA1)	F: 5′-ACG GCA GAT CCA GAT TCA AC-3′ R: 5′-CAG TGC TCA AGC TAC CGTC A-3’	Khan et al. (2016)
	Glycosyltransferase B (gtfB)	F: 5′-AAA ATG GCA CCC ACT TTC TG-3′ R: 5′-TTC TCT CAT CCG TTC TGG CT-3’
	16S Ribosomal RNA (16S rRNA)	F: 5′-CRC CTG GGG AGT RCR G-3′ R: 5′-AGG GTT GCG CTC GTT G-3’
En. faecalis	Gelatinase (gelE)	F: 5′-GGG GCA ATA CAG GGA AAA AT-3′ R: 5′-TCC TTC CCC AGT TTC CTT TT-3’	Lee and Tan (2015)
	Cytolysin activator (cylA)	F: 5′-GAC TCG GGG ATT GAT AGG C-3′ R: 5′-TTT CCA TCT GTC CCA TCC AT-3’
	Serine protease (sprE)	F: 5′-GCG TCA ATC GGA AGA ATC AT-3′ R: 5′-CAT CTT TGG CAT TCG GAT TT-3’
	Collagen adhesin of Enteroccocus (aceE)	F: 5′-GGA ATG ACC GAG AAC GAT GGC T-3′ R: 5′-ATT CGG TTG CGA ACT ATT GG-3’
	16S ribosomal RNA (16S rRNA)	F: 5’-CCG AGT GCT TGC ACT CAA TTG G − 3′ R: 5’-CCT TGG TGA GCC GTT ACC T-3’
Lb. paracasei	Pyruvate oxidase (spxB)	F: 5′-GTG CCG ACG TTA TTT CTT G-3′ R: 5′-ATC ACA ACA ATC GCA GCT C-3’	Reis et al. (2021)
	Pilin protein (spaC)	F: 5′-GGT CAG GGA GAA GCG TAC T-3′ R: 5′-CGG TGT GAC GAC TTA CCA T-3’
	16S ribosomal RNA (16S rRNA)	F: 5′-GTG CTT GCA CCG AGA TTC AAC ATG-3′ R: 5′-TGC GGT TCT TGG ATC TAT GCG-3’