Table 2. Primer and hybridization probe sequences used in the triplex PCR.

Name‡	Primer and probe sequences	Concentration (nM)
OvOo ND5 FW	5’- GCTATTGGTAGGGGTTTGCAT-3’	300
OvOo ND5 Rev	5’- CCACGATAATCCTGTTGACCA -3’	300
16S FW	5’- AATTACTCCGGAGTTAACAGG -3’	500
16S Rev	5’- TCTGTCTCACGACGAACTAAAC -3’	500
Ov probe	TQP 5’ 6-Fam TAAGAGGTTATTGTTTATGCAGATGG 3’ BHQ-1	50
Oo probe*	TQP 5’ Hex TAAGAGATTGTTGTTTATGCAGATAGG 3’ BHQ-1	50
16S probe	TQP 5’ Cy5 TACAACATCGATGTAGCGCAGC 3’BBQ-650	75

‡ Ov = Onchocerca volvulus, Oo = Onchocerca ochengi.