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. 2022 Nov 16;8(46):eadd9468. doi: 10.1126/sciadv.add9468

Fig. 3. TENT-5 deficiency leads to the down-regulation of genes that encode innate immune effectors.

Fig. 3.

(A) RT-qPCR illustrating the induction of tent-5 expression in the wild-type worms challenged with S. aureus at the time point. Relative tent-5 mRNA level was normalized to act-1 and uninfected animals. Data represent means ± SD of three biological replicates; *P ≤ 0.05 (one-tailed t test). (B) MA plot showing differential expression of genes in tent-5(tm3504) mutant compared to wild type upon infection of L4 worms with S. aureus for 8 hours. Plot description as in Fig. 2A. (C) Venn diagrams demonstrating the overlap between genes differentially expressed in tent-5–deficient worms grown on nonpathogenic E. coli and upon infection by S. aureus (FDR < 0.05). MUT, mutant; WT, wild type. (D) RT-qPCR analysis of relative levels of mRNA expression in tent-5(tm3504) and CRISPR-Cas9–generated tent-5(rtt5) mutant grown on E. coli or exposed to S. aureus for 8 hours. Relative abundance of mRNAs was normalized to act-1 and wild type. Data represent means ± SD of three biological replicates; *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 (two-tailed t test).