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. Author manuscript; available in PMC: 2022 Nov 16.
Published in final edited form as: J Proteome Res. 2017 Mar 24;16(4):1693–1705. doi: 10.1021/acs.jproteome.6b01051

Figure 2.

Figure 2.

Validation of the proteins identified as differentially expressed in the iTRAQ experiments. Immunoblotting was used to verify the iTRAQ results in an independent cohort of PS and WS samples collected from pSS and HC donors (n = 5 per group). Overall, significantly higher amounts of β2-microglobulin were observed in pSS PS salivas as compared with HC samples (a), thus verifying the up-regulation detected in the iTRAQ experiment. Down-regulation of carbonic anhydrase VI in PS (b) and BPI fold-containing family B member 2 in WS was also confirmed. Lanes 1–5: pSS; lanes 6–10: HC. Refer to Figure S-1 for images of the entire immunoblot.