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EMSA of triplex formation by morpholino TFOs with all possible structural motifs. The sequences of the TFOs are given in Figure 1. (A) Triplex reactions and the gel were buffered in TBM, pH 7.4. A phosphodiester TFO was included as a positive control. (B) Triplex reactions and the gel were buffered in TAM, pH 5.0. ss, single-stranded DNA; ds, double-stranded DNA; ts, triple-stranded DNA.
