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. 2022 Jun 10;16(6):861–874. doi: 10.5009/gnl210354

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This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — YES1 is a target of miR-129-2-3p. (A) The binding site between YES1 and miR-129-2-3p was analyzed by starBase. (B, C) The relationship between YES1 and miR-129-2-3p was confirmed by dual-luciferase reporter assay. (D, E) The expression of YES1 in tumor tissues (n=66) and normal tissues (n=66) was measured by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. (F) The correlation between YES1 expression and miR-129-2-3p expression in tumor tissues was analyzed by Pearson correlation coefficient. (G, H) The expression of YES1 was measured by qRT-PCR and Western blot in cell lines. (I-K) The expression of YES1 in HGC-27/DR and AGS/DR cells transfected with miR-129-2-3p or anti-miR-129-2-3p at mRNA and protein levels was quantified by qRT-PCR and Western blotting.

YES1, YES proto-oncogene 1; UTR, untranslated region; WT, wild-type; MUT, mutant-type; NC, negative control; DR, doxorubicin; mRNA, messenger RNA. *p<0.05.