5× SDS loading buffer

Reagent	Final concentration	Amount
1 M Tris (pH 6.8)	250 mM	25 mL
SDS	10% (w/v)	10 g
Glycerol	30% (v/v)	30 mL
Bromophenol blue	0.25% (w/v)	0.25 g
2-Mercaptoethanol	5% (v/v)	5 mL
ddH2O	N/A	Up to 100 mL
Total	N/A	100 mL

Aliquot and store at −20°C for several months.