Figure 3.

A representative example ofthe pre-digestion phase of the protocol to liberate intestinal epithelial cells (IECs) at steps 5–7

(A–D) The tissue is cut in two portions of 12 cm² (A, B), transferred to a Falcon tube pre-filled with digestion solution (C, first enzymatic digestion step), and incubated at 37°C for 20 min (D). (E, F) After the enzymatic digestion, wash the tissue (E) and transfer it to a Falcon tube pre-filled with digestion solution (F, second enzymatic digestion step). (G) IECs are visible as a pellet deposit.