Table 5.
Beckman Coulters NaviosEx human mononuclear phagocyte phenotypic panel
| Marker | Fluorochome | Concentration (μL/100 μL)a | Purpose |
|---|---|---|---|
| Viability | APC-Cy7 | 10 | Live cells |
| CD45 | V500 | 5 | Leukocytes |
| CD3 | PerCp-Cy5.5 | 1 | T lymphocytes |
| CD14 | Alexa fluor -700 | 10 | Monocytes/macrophages |
| CD16 | PerCp-Cy5.5 | 1,25 | Monocytes/macrophages |
| CD19 | PerCp-Cy5.5 | 10 | B lymphocytes |
| CD56 | PerCp-Cy5.5 | 2 | NK cells |
| HLA-DR | APC | 5 | Myeloid cells |
| CD11c | PB | 1,25 | Myeloid cells |
| CD123 | PE-Cy7 | 1,25 | pDCs |
| CD64 | PE-CF594 | 5 | Macrophages |
| CD103 | PE | 5 | mDCs |
| CX3CR1 | FITC | 5 | mDCs & macrophages |
a
Add FACS buffer up to 100 μL.