| Reagent | Final concentration | Amount |
|---|---|---|
| PSN | 1% (v/v) | 5 mL |
| Gentamycin (50 mg/mL) | 50 μg/mL (0.001%) | 500 μL |
| RPMI 1640 | N/A | 494.5 mL |
| Total | N/A | 500 mL |
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