| Reagent | Final concentration | Amount |
|---|---|---|
| EDTA (500 mM) | 5 mM (0.01%) | 250 μL |
| DTT (1 M) | 1 mM (0.001%) | 25 μL |
| 1× HBSS-Ca/-Mg | N/A | 24.73 mL |
| Total | N/A | 25 mL |
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