Reagent	Final concentration	Amount
Collagenase VIII (10 mg/mL)	0.25 mg/mL (0.025%)	625 μL
Dnase I (2,000 U/mL)	5 U/mL (0.0025%)	62.5 μL
1× HBSS+Ca/+Mg	N/A	24.31 mL
Total	N/A	25 mL