Reagent | Final concentration | Amount |
---|---|---|
FCS | 5% (v/v) | 50 mL |
PSN | 1% (v/v) | 5 mL |
HEPES (1 M) | 10 mM (0.01%) | 5 mL |
RPMI 1640 | N/A | 440 mL |
Total | N/A | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
FCS | 5% (v/v) | 50 mL |
PSN | 1% (v/v) | 5 mL |
HEPES (1 M) | 10 mM (0.01%) | 5 mL |
RPMI 1640 | N/A | 440 mL |
Total | N/A | 500 mL |