REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD45-V500 (clone HI30) dil. 1/20	BD Biosciences	560779
CD45-PerCp (clone D058-1283) dil. 1/40	BD Biosciences	558411
CD3-PER-CP-Cy5.5 (clone UCHT1) dil. 1/100	BD Biosciences	560835
CD3-V500 (clone SP34-2) dil. 1/33	BD Biosciences	560770
CD8-BV650 (clone RPA-T8) dil. 1/50	BD Biosciences	563821
CD14-Alexafluor700 (clone M5E2) dil. 1/100	BD Biosciences	557923
CD16-PER-CP-Cy5.5 (clone 3G8) dil. 1/80	BD Biosciences	560717
CD16-PE-CF594 (clone 3G8) dil. 1/50	BD Biosciences	562293
CD19-PER-CP-Cy5.5 (clone SJ25C1) dil. 1/10	BD Biosciences	332780
CD20-BV711 (clone 2H7) dil. 1/20	BD Biosciences	563126
CD56-PER-CP-Cy5.5 (clone B159) dil. 1/50	BD Biosciences	560842
HLA-DR APC (clone G46-6) dil. 1/40	BD Biosciences	559866
HLA-DR APC-H7 (clone G46-6) dil. 1/20	BD Biosciences	561358
CD11c-PB (clone 3.9) dil. 1/80	BioLegend	301625
CD11c-APC (clone S-HCL-3) dil. 1/10	BD Biosciences	333144
CD123-PE-Cy7 (clone 7G3) dil. 1/40	BD Biosciences	560826
CD64-PE-CF594 (clone) dil. 1/20	BD Biosciences	565389
CD64-V450 (clone 10.1) dil. 1/20	BD Biosciences	561202
CD103-PE (clone Bly7) dil. 1/20	eBioscience	12-1038
CX3CR1-FITC (clone 2A9-1) dil. 1/20	BioLegend	341605
Biological samples
Human colon samples	IRCCS Ospedale San Raffaele, Milan, Italy	https://www.hsr.it
Macaque colon samples	ICEA, MVA-HB/IDMIT, Fonteney-aux-Roses, France	https://www.idmitcenter.fr
Chemicals, peptides, and recombinant proteins
Deoxyribonuclease (DNase) I from bovine pancreas	Sigma-Aldrich	D4263
Collagenase VIII from Clostridium histolyticum – 500 mg	Sigma-Aldrich	C2139
EDTA 0.5 M, ph 8	Sigma-Aldrich	03690
DL-Dithiothreitol solution (DTT)	Sigma-Aldrich	646563
Gentamycin 50 mg/mL	Gibco- Thermo Fisher Scientific	15750-037
HEPES-buffer solution 1 M	Gibco- Thermo Fisher Scientific	15630-056
Penicillin-Streptomycin-Neomycin (PSN)	Gibco- Thermo Fisher Scientific	15640-055
1× HBSS with calcium chloride and magnesium chloride [HBSS+Ca/+Mg]	Gibco- Thermo Fisher Scientific	24010-091
1× HBSS without calcium chloride and magnesium chloride [HBSS-Ca/-Mg]]	Gibco- Thermo Fisher Scientific	14170-088
1× Roswell Park Memorial Institute 1640 medium (RPMI-1640) + GlutaMAXTM	Gibco- Thermo Fisher Scientific	61870-010
1× Phosphate-buffered saline (PBS)	Gibco- Thermo Fisher Scientific	14190-094
Dimethyl sulfoxide (DMSO) - 100 mL	Sigma-Aldrich	D2650
Ethanol	Various	N/A
Heat-inactivated fetal calf serum (Hi-FCS)	Lonza	DE14-801F
Macaque serum	In-house produced	N/A
BD FACS™ Lysing solution – 100 mL	BD Biosciences	349202
Critical commercial assays
LIVE/DEADTM Fixable Blue Dead Cell Stain kit	Thermo Fisher Scientific	L23105
LIVE/DEADTM Fixable Near-IR for 633 or 635 excitation Dead Cell Stain kit	Thermo Fisher Scientific	L10119
Software and algorithms
BD FACSDiva	BD Biosciences	https://www.bdbiosciences.com
FlowJo	BD Biosciences	https://www.flowjo.com
Deposited data
FCS raw data	FlowRepository	http://flowrepository.org/id/FR-FCM-Z5Q9
Other
Scissors	Coveto	804568
Forceps	Coveto	719328
50 mL FALCON tubes	Fisher Scientific	352070
Falcon® 100 mm TC-treated Cell Culture Dish (100 mm × 20 mm Petri dish)	Corning Life Sciences	353003
Falcon® 150 mm × 15 mm Not TC-treated Bacteriological Petri Dish (150 mm × 15 mm Petri dish)	Corning Life Sciences	351058
0.45-μm filter unit, 200 mL	Thermo Fisher Scientific	126-0045
70-μm cell strainer	Corning Life Sciences	352350
CorningTM 10 mL serological pipettes	Fisher Scientific	356551
CorningTM 25 mL serological pipettes	Fisher Scientific	356525
Cryovials 1.8 mL	Thermo Fisher Scientific	375418PK
FACS tubes 5 mL	Corning Life Sciences	352063
96-well plate U-bottom	Corning Life Sciences	353077
Corning® CoolCell™ LX Cell Freezing Container	Sigma-Aldrich	CLS432001
VorTemp 1550 shaking incubator	Labnet International	S2050A
LSRFortessa flow cytometer	BD Biosciences	N/A
Beckman Coulters NaviosEx flow cytometer	Beckman Coulter	N/A