Fig. 5.

RSL-MOA shows dose-dependent binding to H1299 cells. A) Representative histogram plot of gated living H1299 cells pre-incubated with fluorescently labeled RSL-MOA-Cy5. Histograms of fluorescence intensity reveal a dose-dependent trend in binding according to used lectin concentrations. B) Representative histograms of fluorescence intensity of H1299 treated with RSL-MOA-Cy5 pre-incubated with 100 mM fucose. Inhibition of the RSL domain with high concentrations of fucose determines a shift in fluorescence intensity towards lower values (pink histogram), suggesting a reduced binding of lectin to H1299 cells. C) Representative histograms of fluorescence intensity of H1299 treated with RSL-MOA-Cy5 pre-incubated with 100 mM PNPG do not show a significant reduction in lectin binding to the cell surface. The control experiments correspond to samples of H1299 cells without any addition of lectin sample. The number of cells within the live population (y-axis) is plotted against the fluorescence intensity of RSL-MOA-Cy5 (x-axis). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)