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Southern blot analysis of mtb32a genomic sequences. One microgram each of genomic DNAs from several strains of the M. tuberculosis complex (H37Ra, H37Rv, Erdman, and C) and BCG and from other environmental mycobacterial species (M. scrofulaceum, M. smegmatis, M. fortuitum, M. malmoense, and M. gordonae) was digested with PstI and probed with a 600-bp coding fragment of MTB32A. Numbers at left are the sizes, in kilobase pairs, of HindIII-HincII-digested DNAs.
