Fig. 1.
Optimization of the protocol for elution-free MB-based method. Effect of (a) the MB size, (b) the MB dosage, (c) the PEPPG F68 concentration in lysis buffer, (d) the incubation time for MBs and samples in lysis buffer, (e) the NaOH concentration in washing buffer, and (f) the sample volume applied for nucleic acid extraction on the efficiency of LAMP assays