Table 1.
Pathological grading of inflammation severity of lung tissue after high- and re-dosing of AAVs
| Inflammation severity (grade 0–5) | Single dose of AAV6-CAG-GFP or mCherry (at week 1 after dosing) |
1–2 doses of AAV6-SpCas9 and AAV6-LSL-sgRNApair-GFP (2 × 1012 vg/mouse/dose, at week 2 after the first doing) |
3 doses of AAV6-Notch2-sgRNApair-mCherry (4 × 1012 vg/mouse/dose, at weeks 4 or 8 after the first dosing) |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 8 × 1012 vg/mouse, n (%) | 1.2 × 1013 vg/mouse, n (%) | PBS control, n (%) | 1 dose, n (%) | 2 doses, n (%) | PBS, n (%) | Week 4, n (%) | Week 4 PBS, n (%) | Week 8, n (%) | Week 8 PBS, n (%) | |
| Column ID | A | B | C | D | E | F | G | H | I | J |
| Grade 0 | 3 (27.3) | 7 (63.6) | 1 (50) | 11 (91.7) | 7 (77.8) | 3 (100) | 3 (21.4) | 3 (100) | 6 (46.2) | 2 (100) |
| Grade 1 | 4 (36.4) | 2 (18.2) | 1 (50) | 1 (8.3) | 2 (22.2) | 0 | 7 (50) | 0 | 6 (46.2) | 0 |
| Grade 2 | 2 (18.2) | 2 (18.2) | 0 | 0 | 0 | 0 | 4 (28.6) | 0 | 1 (7.6) | 0 |
| Grade 3 | 2 (18.2) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Grade 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Grade 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |